About

This ELISA kit uses Indirect ELISA as the method to qualitatively detect theMonkeypox Virus (MPXV) IgG in the sample. The micro ELISA plate provided inthis kit is pre- coated with purified MPXV antigen, after adding samples to wells, the MPXV IgG in the samples will combine with the pre-coated MPXV antigen. Afterwashing completely, add Horseradish Peroxidase (HRP) conjugated goat anti humanIgG to develop the antigen antibody HRP conjugated secondary antibody complex. Free components are washed away, then the substrate solution is added to each well. Only those wells that contain MPXV IgG and HRP conjugated anti human IgG willappear blue in color. The enzyme- substrate reaction is terminated by the addition ofstop solution and the color turns yellow.The optical density (OD) is measured spectrophotometrically at a wavelength of 450 + - 2 nm. Compared with the CUT OFF value to judge whether MPXV IgG exists inthe tested samples or not.